Cloning Enzyme Ab initio Sequencing

Background

Cloning Enzyme Ab initio Sequencing

Cloning enzyme molecules play a key role in the development of biopharmaceuticals such as monoclonal antibodies, biological vaccines, and diagnostic kits. Creative Enzymes has built a new generation of ab initio sequencing platform based on high resolution mass spectrometry combined with extensive experience in bioinformatics analysis, which enables fast and accurate analysis of monoclonal antibody primary structure, protein sequences and mutations.

Analysis Approaches We Offer

Cloning Enzyme Ab initio Sequencing

1. Full sequence coverage

During the sequence determination process we will select 5 different proteases for enzymatic cleavage and use the complementarity between different enzymatic cleaved peptides to achieve 100% full sequence splicing of the enzyme molecule.

2. Advanced data processing algorithm

We developed our own raw data calculation method for mass spectrometry, using the principle of wide in and narrow out, to achieve accurate identification of protein sequences without missing any valid data information.

3. High precision and accuracy

We use high resolution and high sensitivity mass spectrometers in the sequencing process.

4. Rigorous sequence prediction and strict sequence verification

We will first perform sequence prediction on the sequencing results, and at the same time, we will build a new database with the determined sequences, and analyze the collected mass spectrometry data again by sequence confirmation mode, and we will only provide the customers with the results that match perfectly.

5. Sequencing and mutation analysis

Based on our high-resolution mass spectrometer combined with our rich experience in bioinformatics analysis, we have established a new generation of ab initio sequencing platform for rapid and accurate analysis of protein primary structures.

Service Advantages

  1. Using high resolution mass spectrometer.
  2. High sample coverage: up to 100% coverage.
  3. High first amino acid accuracy: up to 95% accuracy.
  4. Low sample volume: only 100 ug of sample is required to complete the assay.

Our Features

  • We have accumulated many years of essence in the field of cloning enzyme ab initio sequencing service, helping customers to speed up project development and improve the overall success rate of the project.
  • At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.
  • Creative Enzymes has in-depth cooperation with many biology companies and research institutes, and has extensive experience in providing them with high-quality products and services.

Service Process

Service Process

Deliverables

  • Results raw data
  • Data analysis
  • Results analysis
  • Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a company that provides professional and comprehensive cloning enzyme Ab initio sequencing service. We have years of experience to meet your specific project needs. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.

Inquiry

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