Overview

With the continuous development and improvement of classical methods, various chemical modifications based on mass spectrometry and enzyme-assisted techniques, the N-terminal sequence analysis of proteins and peptides has obtained abundant terminal peptide sequence information, which provides a strong basis and acceleration for the identification of proteins and peptides. The study of advanced structures and modification sites of protein drugs. N-terminal sequencing analysis techniques for thousands of proteins in complex biological systems remain a great challenge for us, especially for large-scale more detailed determination of N-terminal modification diversity, and more targeted research strategies are needed.

Service Process

Enzyme N-terminal Sequence Methods

Edman degradation

Edman degradation is a very mature and classic method for N-terminal sequencing of proteins and peptides, and is widely used in the field of biotechnology. The Edman degradation method has been widely used as the gold standard for N-terminal sequence testing of existing protein samples. It is a valuable research tool for N-terminal sequence analysis of the entire purified protein and the most reliable sequencing method.

Mass spectrometry analysis

Mass spectrometry-based N-terminal sequence sequencing techniques can simultaneously determine protein N-terminal sequences, especially electrospray ionization (ESI) and matrix-assisted laser desorption/ionization time-of-fight (MALDI-TOF), which have revolutionized the application of mass spectrometry in protein structural analysis. High-sensitivity, high-precision, high-resolution, high-throughput biomass spectrometry provides an important option for protein N-terminal sequencing.

Chemical labeling combined with mass spectrometry

Many research methods for N-terminal peptides use a combination of mass spectrometry technology and a variety of chemical methods and biological enzymatic methods. For example, the protein is blocked by reduction, alkylation and guanidylation of side chain amino groups. The free N-terminal is labeled with different biotin reagents. After the labeled protein is digested with trypsin, the labeled N-terminal peptide is separated by the avidin affinity system, and then passed through MALDI-TOF/MALDI-TOF-PSD MS de novo sequencing to obtain the sequence of N-terminal peptide.

Our Features

• For each custom project, we assign a team of expert chemists and biologists to help our partners define their research path and goals.
• We also provide full assistance in realizing the scientific and business plan, as well as delivering the scientific and business plan.
• Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in DNA modifying enzyme service.

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

At Creative Enzymes, our goal is to apply innovative technologies to the production.We offer innovative, flexible and scalable solutions that can meet the needs of our customers. We strive to meet this challenge as a global company that places a high priority on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality. If you would like to know more about this service, please feel free to contact us.