Overview

Restriction enzymes are important tools for manipulating DNA molecules. These enzymes cleave DNA at specific sites, producing fragments that can be separated and analyzed by gel electrophoresis.SpeI is a type II restriction enzyme that recognizes the sequence "ACTAGT" and cleaves between the A and T nucleotides of the upper strand and between the G and A nucleotides of the lower strand.SpeI is commonly used in recombinant DNA technology to create compatible ends for ligation reactions and for cloning and sequencing studies to generate DNA fragments.

Sepecific Services We Offer

Creative Enzymes is a leading provider of restriction enzyme SpeI services to researchers in the biotechnology industry. Our team of experts  has extensive experience in the production, purification and characterization of enzymes, including SpeI, which is frequently used in molecular biology applications. At Creative Enzymes, we offer a comprehensive SpeI service package that includes enzyme production, purification and characterization. Our team uses advanced technologies to optimize SpeI expression in bacterial or yeast systems, ensuring high yields of active enzymes. The purified SpeI is characterized by SDS-PAGE and activity assay to confirm its purity and function.

Workflow

• Production: We produce SpeI in bacterial or yeast systems using a proprietary protocol. customers can choose from multiple expression hosts and vectors based on their specific needs.
• Purification: After producing SpeI, our team performs a series of purification steps to separate the enzyme from other proteins and contaminants. We utilize various chromatographic methods such as ion exchange, affinity and size exclusion chromatography to achieve high purity levels.
• Characterization: We use SDS-PAGE and activity assays to assess the purity and functionality of the purified SpeI. Our team ensures that the enzyme is free of nucleases, proteases and other impurities that could interfere with downstream applications.

Sample Requirements

• In order to perform SpeI services, we need at least 1 µg of plasmid DNA or 10 µg of genomic DNA.
• DNA should be of high quality and purity, as contaminants can affect enzyme performance. Clients can submit their samples in various forms including lyophilized powder, frozen pellets or liquid solutions.
• We recommend that customers consult with our team prior to submitting samples to ensure proper handling and storage.

Deliverables

• At Creative Enzymes, we provide our customers with a comprehensive results report detailing the production, purification and characterization of SpeI.
• The reports include information on the yield, purity and activity of the enzyme, as well as any technical details related to the production process.
• Customers can receive the reports in electronic format and we can also provide custom data analysis and interpretation services upon request.

Why Choose Us?

Creative Enzymes is a leading provider of SpeI services to researchers in the biotechnology industry. With our expertise in enzyme production, purification and characterization, we can provide high quality SpeI for a variety of molecular biology applications. our team is committed to providing customized services to meet the unique needs of each project, and we pride ourselves on delivering results in a timely and cost-effective manner. Contact us today to learn more about our SpeI services and how we can support your research.