Overview

DNA modifying enzymes are key to many biotechnological applications such as gene therapy, drug discovery and genetic engineering. To study DNA modifying enzymes, researchers often use affinity purification assays to isolate these enzymes from complex mixtures of cell extracts. DNA modifying enzyme affinity purification assays are a powerful technique that allows researchers to identify and quantify DNA modifying enzymes in biological samples with high specificity.

Methods

DNA modifying enzyme affinity purification assays provide a powerful tool for studying DNA modifying enzymes in biological samples. This assay allows researchers to isolate and identify enzymes with high specificity, providing valuable insights into their biology and function. Our services provide the most advanced technology and expertise in the field, enabling us to deliver reliable and accurate results to our clients.

Sepecific Assay Service We Offer

At Creative Enzymes, our team of experts has extensive experience in the design, optimization and execution of these assays, using a variety of ligands and solid supports to isolate and quantify DNA-modifying enzymes in biological samples. Our services include the following steps:

• Sample Preparation

We provide advice and protocols for sample preparation to ensure that your samples are compatible with the assay.

• Ligand Immobilization

We immobilize selected ligands on a solid support such as beads or columns to create an affinity matrix.

• Affinity purification

Sample extracts are incubated with the affinity matrix so that the target DNAx-modifying enzyme binds specifically to the ligand, while non-specific proteins are washed away.

• Elution and Quantification

Finally, the enzyme is eluted from the matrix and its activity or quantity is measured using various techniques such as enzymatic assays or mass spectrometry.

Sample Requirements

• Our affinity purification assay service requires a minimum of 1 mg of protein extract per sample.
• Samples should be prepared in a buffer that is compatible with the ligand used in the assay.
• We recommend consulting with our team to ensure that your sample preparation is suitable for the assay.

Applications

Our Features

• Customized assay design and optimization
• Expertise in various ligands and solid supports
• High throughput screening and analysis
• Data analysis and interpretation
• Ongoing consulting and support

Deliverables

• Raw data: We provide all raw data generated during the assay, including sample spectra, chromatograms, and other relevant data.
• Assay protocol
• Sample preparation details
• Data analysis and interpretation
• Consulting: Our team provides ongoing consulting and support to ensure that your research goals are met.

Why Choose Us?

Creative Enzymes is a professional DNA modifying enzymes affinity purification assay provider supporting a wide range of enzyme services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. We have accumulated years of quintessence in the field of DNA modifying enzymes affinity purification assay, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.