Where do Restriction Enzymes Work?

Restriction endonucleases specifically cut the phosphodiester bond in the DNA strand, DNA polymerases use a DNA strand as a template to form a single nucleotide through a phosphodiester bond to form a DNA strand complementary to the template strand, DNA ligases join two gaps in the DNA double strand at the same time DNA ligases do not require a template, deconvolutive enzymes: are a class of enzymes that unwind hydrogen bonds.DNA restriction enzymes DNA restriction enzymes act on phosphodiester bondsDNA ligases act on phosphodiester bonds DNA polymerases act on phosphodiester bondsDNA deconvolutive enzymes act on hydrogen bonds.

Methods

Target prediction uses specific computational methods to predict the potential targets or pharmacological effects of a compound. Commonly used methods include those based on directional docking and those based on molecular fingerprinting to calculate similarity.

• Reverse docking

The reverse docking technique relies on the crystal structure of the protein. However, the current prediction accuracy is low due to the inaccuracy of the scoring function.

• Method based on molecular fingerprint

The similarity method based on molecular fingerprint is widely used due to the fast calculation speed. Its accuracy needs to be improved because only the topological structure is considered and the three-dimensional properties are ignored.

Sepcific Restriction Enzyme Target Prediction Process We Offer

• Step 1: Establishing a database and depositing data on the restriction enzyme targets and their corresponding ligands into the database.
• Step 2: Generating three-dimensional conformations of ligand molecular structures based on molecular force fields.



• Step 3: editing the chemical structure of the molecule to be predicted.
• Step 4: generating the three-dimensional conformation of the molecule to be predicted in step 3 based on the molecular force field corresponding to step 2.
• Step 5: analyzing the three-dimensional conformation of the molecule to be predicted and the three-dimensional conformation of the ligand molecular structure in the database.
• Step 6: calculating the matching degree based on the analysis results, outputting the data of drug targets corresponding to ligands whose matching degree exceeds a preset threshold, and obtaining a list of drug targets of the molecule to be predicted.

Service Process

Our Features

• Creative Enzymes has established a proven enzyme technology platform for a variety of applications. We'll help you design the right restriction enzyme enzyme target prediction to aid your scientific research.
• Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in restriction enzyme enzyme target prediction service.

Deliverables

• Related prediction results raw data
• Data analysis
• Reverse docking results
• Image and result analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a professional restriction enzyme target prediction service provider supporting a wide range of enzyme services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. We have accumulated years of quintessence in the field of restriction enzyme service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.