Background

The purity and homogeneity of cloning enzyme are essential for enzyme crystallization as well as rigorous structural and functional studies. Impure samples can lead to inaccurate results throughout the experiment. In addition, cloning enzyme purity and homogeneity are two very important characteristics for further protein research and biopharmaceutical applications. Common contaminants of cloning enzyme products include media, reagents in extraction and purification steps, etc. In addition to impurities, non-homogeneity of cloning enzyme may be due to different intracellular reactions as well as post-translational modifications. It is important to analyze purity and homogeneity before further processing of the cloning enzyme.

Analysis Approaches We Offer

Creative Enzymes offers a range of services for cloning enzyme purity and homogeneity characterization to detect contaminants, protein variants, heterodimers, S-S chain mismatches, incomplete protein products (truncated proteins), degraded proteins, protein modifications, protein aggregates and protein precursors. Free consultation is welcome!

• Purity and integrity analysis methods: SDS-PAGE, capillary electrophoresis, zinc reverse staining, glutaraldehyde-free modified silver stain.



• Homogeneity analysis: dynamic light scattering, UV-Vis fluorescence spectroscopy, size exclusion chromatography, static light scattering.

Sample Requirements

We can process samples of antibodies, antigens, enzymes, growth factors, DNA binding proteins, blood proteins, membrane proteins, etc. Common samples include

• Proteins of natural origin
• Fusion and marker proteins of recombinant origin
• Antibodies of different isoforms
• Membrane proteins

Service Advantages

• Advanced technology platforms: we are equipped with a variety of technology platforms to accurately determine purity and homogeneity.
• Accuracy and reproducibility: we provide accurate and detailed results regarding sample purity and homogeneity, thus saving time wasted on bad samples.
• Wide range of applications: purified and homogenized proteins can be used for research and medical applications, such as development and quality control of therapeutic proteins.

Our Features

• Creative Enzymes will complete your project on time and efficiently.
• Creative Enzymes works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
• Creative Enzymes has a proven, advanced technology platform that is constantly being improved to ensure that we can provide accurate, sensitive, and rapid analytical results for our customers.

Service Process

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a biotechnology company that specializes in providing tool enzyme services. As a reliable supplier, Creative Enzymes offers its services with high quality and competitive price/performance ratio. Creative Enzymes has accumulated years of quintessence in the field of purity and homogeneity characterization service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.