DNA methyltransferases (DNMTs) are enzymes that add methyl groups to the cytosine residues of CpG dinucleotides, thereby regulating gene expression and other biological processes. Aberrant DNMT activity has been associated with a number of diseases, including cancer.DNMT inhibitors are potential drug candidates for the treatment of these diseases, and screening for effective inhibitors is key to drug discovery.Creative Enzymes provides expert services in DNA methyltransferase inhibitor screening to help researchers identify effective inhibitors for their studies.
Enzyme activity assay: The assay uses techniques such as mass spectrometry or fluorescent labeling to detect changes in methylation patterns.
Cell-based assays: Cell-based assays use cellular models to assess the inhibitory effect of the test compound on DNMT activity.
Computational methods: Computational methods use molecular docking simulations and virtual screens to predict potential DNMT inhibitors based on their binding affinity and structural properties.
We require a minimum of 1 μg of purified enzyme or cell lysate per assay. We recommend that samples be frozen at -80°C until shipment.
We provide our clients with detailed reports that include all experimental procedures and results obtained. The report includes a summary of the assay design, optimization, validation and data analysis.
Creative Enzymes has a state-of-the-art facility equipped with the latest technology for DNA methyltransferase inhibitor screening services. Our dedicated team includes senior scientists with expertise in molecular biology, biochemistry and biotechnology. We use proven and optimized protocols to ensure accurate and reproducible results. We are committed to providing high quality services that meet our clients' needs and exceed their expectations.
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