Creative Enzymes offers DNA modifying enzyme antibody label binding services for signal generating molecules. Various reporter enzymes, such as horseradish peroxidase (HRP), alkaline phosphatase (AP), etc., can be attached to antibodies and proteins using different coupling chemicals to ensure maximum retention of enzyme and protein activity. In addition, non-enzymatic fluorescent markers and biotin can be coupled to specific antibodies, proteins and peptides as a means of signal generation. In addition to DNA modifying enzyme antibody markers, we also offer:
These enzyme-antibody couples can be used in the following applications: enzyme-linked immunosorbent assays (ELISA), blotting techniques, in situ hybridization, cytochemical and histochemical detection systems.
Our enzyme-antibody conjugates are prepared by techniques that yield an enzyme to target protein ratio of approximately 1:1, thus ensuring maximum retention of enzyme and protein activity. Since each antibody/protein has its own unique characteristics, the conjugation needs to be optimized prior to the start of the binding process. Creative Enzymes will assign a dedicated project manager to evaluate and gather a specific project scope to ensure fast, efficient and optimized antibody-enzyme conjugates.
Antibody purity should be greater than 95%. Antibodies should be provided at a minimum concentration of 5 mg/ml in neutral buffer without preservatives, protease inhibitors, or carrier proteins. If reagents are provided and presented by the customer, a certificate of quality is required. If no proof of quality is available, Creative Enzymes also assist with affinity chromatography services using the appropriate immobilized antigen or immunoglobulin binding protein.
Creative Enzymes is a professional DNA modifying enzyme labeling service provider supporting a wide range of enzyme services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. We have accumulated years of quintessence in the field of DNA modifying enzyme labeling service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.
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