Restriction enzymes recognize short DNA sequences and cut double-stranded DNA at specific sites within or adjacent to these sequences. approximately 3,000 restriction enzymes have been identified that recognize over 230 different DNA sequences. They are found primarily in bacteria, but have also been isolated from viruses, archaea and eukaryotes. It is estimated that 25% of all bacteria contain at least one restriction enzyme, and up to seven restriction enzymes have been found in a single species. The characterization of restriction enzymes is crucial. Creative Enzymes provides you with a variety of restriction enzyme characterization services to meet your scientific research needs.
Analytical characterization methods are necessary to ensure the identity, purity, structural and conformational integrity, and activity of the restriction enzyme product. Enzyme assays are performed within the scope of measuring key benchmarks. We offer the following restriction enzyme characterization services for you to choose from:
Restriction Enzyme Characterization Services | Methods |
Evaluation of restriction enzyme purity | SDS-PAGE |
Measurement of restriction enzyme concentration | HPLC: Determine the identity, content and purity of the enzyme. sEC-HPLC can be used to determine the degree of aggregation. |
Restriction Enzyme identification by mass spectrometry | ELISA: Specific identification of the enzyme by antibodies. |
Measurement of restriction enzyme molecular weight | bBCA assay: Uses bicarbonyl urea to react with BCA to produce a purple product that is used to quantify the enzyme. |
Crystallization Services | A variety of enzyme crystallization techniques are available, including saline crystallization, organic solvent crystallization, dialysis equilibrium crystallization, complex crystallization, and isoelectric point crystallization. |
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Creative Enzymes is a company that provides professional and comprehensive restriction enzyme characterization services. We have years of experience to meet your specific project needs in using restriction enzyme characterization services research. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.
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