Creative Enzymes has developed an efficient screening system to select inhibitors by detecting fluorescent, chemiluminescent or isotopic signals. Typically, candidate molecules can be selected or synthesized and then immobilized on a solid surface. The assay is performed after incubation with the target enzyme, depending on the specific experimental design.

Sepcific Restriction Enzyme Inhibitor Screening Service Approaches We Offer

Creative Enzymes has developed a range of assays for screening restriction enzyme inhibitors specifically for lead discovery. Our enzyme inhibitor screening services include, but are not limited to, determining the EC₅₀ or IC₅₀ and the mode of action of the lead compound.

Our services include, but are not limited to

• Determining Km of restriction enzymes

Creative Enzymes performs a substrate titration on your restriction enzyme of interest and then analyzes it using mapping software to determine Km. Once Km is determined, that substrate concentration can be used in the inhibitor screening assay.

• Measuring solvent interference

To test for solvent interference, Creative Enzymes titrates the solvent with the enzyme, looking for any increase or decrease in enzyme activity. If there is an interference, we determine the solvent concentration at which the interference becomes negligible. This solvent concentration is then used for the inhibitor screening test.

• Determining inhibitor IC₅₀

The inhibitor titrated in the solvent is incubated with the enzyme of interest. After incubation with the inhibitor, the activity of the enzyme is measured using the Km of the substrate concentration. Graphing software was used to plot the inhibition data and to determine the IC₅₀ of the inhibitor.

Applications

• Quantitative evaluation of enzyme inhibitors
• Accurate batch-based measurement and comparison of inhibitor candidates
• High-throughput screening to search pre-built libraries containing > 10,000 candidates
• Both surface-based and solution-based assays available
• Enzyme analysis in disease states or under different treatments (drugs, growth factors, hormones, etc.)

Service Process

Our Features

• We have accumulated many years of essence in the field of restriction enzyme inhibitor screening service, helping customers to speed up project development and improve the overall success rate of the project.
• At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.
• Creative Enzymes has in-depth cooperation with many biology companies and research institutes, and has extensive experience in providing them with high-quality products and services.

Deliverables

• Related results raw data
• Data analysis
• Image and result analysis
• Results analysis
• Screening report
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a company that provides professional and comprehensive restriction enzyme inhibitor screening service. We have years of experience to meet your specific project needs. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.