Psr I | Creative Enzymes

Psr I

Cat#	Product Name	Recognition Sequence	Size	Price	Qty	Inquiry
ET-1160RE	Psr I	↑(N)7GAACNNNNNNTAC(N)12↑ ↓(N)12CTTGNNNNNNATG(N)7↓	100U	$ 489		Add To Cart
		↑(N)7GAACNNNNNNTAC(N)12↑ ↓(N)12CTTGNNNNNNATG(N)7↓	500U			Inquiry

Cat :

ET-1160RE

Recognition Sequence :

↑(N)7GAACNNNNNNTAC(N)12↑
↓(N)12CTTGNNNNNNATG(N)7↓

Unit Definition :

One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 30°C in a total reaction volume of 50 μl.

Reaction Temperature :

30°C

Form :

Liquid

Storage Buffer :

10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol.

Ligation :

After 2-fold overdigestion with enzyme more than 70% of DNA fragments can be ligated. Of these 80% can be recut. In the presence of 10% PEG ligation is better.

Source :

Pseudomonas stutzeri N2

Assayed on :

T7 DNA

Working buffer :

Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Non-specific hydrolisis :

No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 30°C.

Size :

100U; 500U

Concentration, u.a./ml :

1000-2000

Inactivation :

20min Under 65°C

Reagents Supplied :

10 X SE-buffer Y, BSA

Storage :

-20°C

Notes :

High enzyme concentration may result in star activity.
Incubation at 37centigrade results in 20% activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.

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