Recognition Sequence :
YA↑TR
RT↓AY
Unit Definition :
One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the above indicated structure in 1 hour at 50°C in a total reaction volume of 20 μl.
Reaction Temperature :
50°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol
Ligation :
After 3-fold overdigestion with enzyme about 90% of the pUC19 DNA fragments can be ligated with DNA Ligase and recut.
Source :
Flavobacterium aquatile B15
Assayed on :
Double-stranded oligonucleotide
5`- CGAGTTCA^TAGCTGGGCCCAAC -3`
3`- GCTCAAGT^ATCGACCCGGGTTG -5`
Working buffer :
B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Concentration, u.a./ml :
2000
Inactivation :
20min Under 80°C
Reagents Supplied :
10 X SE-buffer B
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