Recognition Sequence :
↑(N)7GAAGNNNNNNTAC(N)12↑
↓(N)12CTTCNNNNNNATG(N)7↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
20 mM KH2PO4(pH 7.4); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol.
Ligation :
After 2-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 95% can be recut.
Source :
Bacillus sphaericus
Working buffer :
SE-buffer 2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
500-2000 500-2000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer 2K
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