Recognition Sequence :
CC↑TCGAGG
GGAGCT↓CC
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19SE/DriI -digest in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol
Ligation :
After 2-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Source :
Arthrobacter species 7M06
Assayed on :
pUC19SE/DriI - digest
Working buffer :
SE-buffer AbsI (10 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 50 mM KCl; 1mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
500-1000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer AbsI
Notes :
A long incubation time may result in star activity.
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