Recognition Sequence :
GAC↑GTC
CTG↓CAG
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol.
Ligation :
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better.
Source :
An E.coli strain that carries the cloned Zra I gene from Zoogloea ramigera 11
Working buffer :
B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
10000
Inactivation :
20min Under 80°C
Reagents Supplied :
10 X SE-buffer B
Notes :
High enzyme concentration may result in star activity. The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,5. ZraI cleaves linear plasmid DNA at a rate 1,5-2 times higher than supercoiled plasmid DNA.
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