Recognition Sequence :
C↑CCGGG
GGGCC↓C
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus -2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
20 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol.
Ligation :
After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated. Of these 90% can be recut.
Source :
An E.coli strain, that carries the cloned gene XmaI from Xanthomonas malvacearum
Assayed on :
Adenovirus -2 DNA
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 37°C
Concentration, u.a./ml :
3000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer Y
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