Product Description :
The vaccinia virus capping system uses the vaccinia virus capping enzyme and related components to add the 7-methylguanosine cap structure (m7Gppp, Cap 0) to the 5'end of the RNA. In eukaryotes, this structure is closely related to the stability, transport and translation of mRNA. Enzymatically capping RNA is a simple and effective method, which can significantly improve the stability and translation ability of in vitro transcribed RNA for transfection and microinjection. The vaccinia virus capping enzyme consists of two subunits (D1 and D12). The D1 subunit performs the functions of RNA triphosphatase and guanosine transferase, and the D12 subunit performs the functions of guanine methyltransferase.
Components :
• Vaccinia Capping Enzyme (10 U/μl)
• 10 x Capping Buffer
• GTP (10 mM)
• SAM (32 mM)
• RNase-Free Water
Unit Definition :
1 Unit is defined as the amount of enzyme required to incorporate 10 nmol of [α-32P] GTP into a 80-nucleotide (80 nt) transcript within 1 hour under the conditions of 37°C and pH 8.0
Storage Buffer :
50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100 and 50% Glycerol (25℃, pH 8.0).
Concentration, u.a./ml :
10 U/μl
Our Products Cannot Be Used As Medicines Directly For Personal Use.
Protocol