Recognition Sequence :
GACN↑NNGTC
CTGNN↓NCAG
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 65°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 500 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol.
Ligation :
After 2-fold overdigestion with enzyme 10% of the DNA fragments can be ligated.
Source :
An E.coli strain, that carries the cloned gene Tth111I from Thermus Thermophilus 111
Assayed on :
Lambda DNA (HindIII-digest)
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C.
Concentration, u.a./ml :
5000
Inactivation :
20min Under 80°C
Reagents Supplied :
10 X SE-buffer Y
Notes :
High enzyme concentration may result in star activity.
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