Recognition Sequence :
T↑TAA
AAT↓T
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Reaction Temperature :
65°C
Storage Buffer :
10 mM Tris-HCl-(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol.
Ligation :
After 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Source :
An E.coli strain that carries the cloned Tru9 I gene from Thermus ruber 9
Working buffer :
W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65°C.
Concentration, u.a./ml :
20000
Inactivation :
20min Under 80°C
Methylation sensitivity :
Blocked by TTAmA methylation .
Reagents Supplied :
10 X SE-buffer W
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