Product Description :
TEV Protease (His-tag) is a cysteine protease of Tobacco Etch Virus (TEV) with a His tag (6X His tag) recombinantly expressed in Bacillus subtilis, which can specifically recognize Heptapeptide sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser, and enzyme cleavage between Gln and Gly/Ser amino acid residues, commonly used to remove Glutathione S-transferase (GST), His or other tagged proteases. 1000U TEV Protease (His-tag) is sufficient for the cleavge of 3mg tagged protein.
Components :
·TEV Protease (His-tag) (1U/µl)
·10X TEV Buffer
Unit Definition :
The amount of enzyme required to cleave 3 µg of the control substrate to more than 85% after 1 hour of reaction at 30°C and pH 8.0 is one activity unit.
Storage Buffer :
25mM Tris-HCl, 150mM NaCl, 1mM EDTA, 5mM DTT, 50%(v/v)glycerol, pH8.0
Concentration, u.a./ml :
1U/µl
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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