TA DNA Polymerase is a mixture of Taq DNA Polymerase with a proofreading 3’-5’ exonuclease. The fidelity is equal to HiFi Pfu DNA Polymerase. The yield is equal to that from Taq DNA Polymerase. It is more suitable for high fidelity TA cloning. TA DNA Polymerase offers 18-fold fidelity as compared to Taq DNA Polymerase. Extension rate is about 1-2 kb/min. Template-independent“A”can be generated at the 3’end of the PCR product. Amplification of genomic DNA fragment up to 8 kb.
PDF