T7 RNA Polymerase

Cat :
GMP-001
Product Description :
T7 RNA Polymerase (T7 RNA polymerase) is a protein encoded by phage T7 DNA expressed by recombinant E. coli. It is a DNA-dependent 5'→3' RNA polymerase that recognizes the T7 promoter sequence with high specificity. This product catalyzes the synthesis of RNA complementary strand to the single-stranded DNA or double-stranded DNA template strand downstream of the promoter.
Components :
·T7 RNA Polymerase(50U/μl)
·Transcription Buffer(10X)
Unit Definition :
The amount of enzyme required to incorporate 1 nmol of [3H]ATP into an acid-insoluble precipitate in 1 hour at 37°C, pH 8.0 is defined as 1 activity unit.
Storage Buffer :
50 mM Tris-HCl (25℃, pH7.9),100 mM NaCl, 0.1 mM EDTA, 2 mM DTT, 0.1% Triton X-100 and 20% Glycerol.
Concentration, u.a./ml :
50U/μl
Storage :
Store at -20℃
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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