sgRNA Synthesis Kit

Cat :

CE-3510

Product Description :

This product is designed to synthesize gRNAs in vitro. In the bacterial CRISPR/Cas9 system, the Cas9 nuclease associates with two RNAs, the CRISPR RNA (crRNA) and the trans-activating crRNA (tracrRNA), to direct sequence-specific DNA cleavage. The gRNA (guide RNA) is a fusion of the natural crRNA and tracrRNA components. It contains an 18-20 base variable sequence that can be changed to target any DNA sequence that is adjacent to an NGG proto-spacer adjacent motif (PAM) on the 3’ end of the target sequence. The sgRNA synthesis kit allows user to fuse their choice of 18-20 base target sequence within the gRNA DNA sequence provided, by PCR fusion. The kit also provides materials for in-vitro transcription of the generated gRNA DNA in order to generate gRNAs which can be directly used for in-vivo genome editing.

Storage Buffer :

10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol PH 7.4, at 25°C

Source :

E.coli

Size :

20 reactions/kit

Storage :

Store at -20 ℃

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