Recognition Sequence :
C↑TCGAG
GAGCT↓C
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (Hind III-digest) in 1 hour at 50°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Ligation :
After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Source :
Streptomyces fradiae 274
Assayed on :
Lambda DNA (Hind III-digest)
Working buffer :
B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C.
Concentration, u.a./ml :
20000
Inactivation :
20min Under 65°C
Methylation sensitivity :
Blocked by CTCGmAG methylation.
Not blocked by CTmCGAG methylation.
Reagents Supplied :
10 X SE-buffer B.
Notes :
Incubation at 37°C results in 70% activity
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