Sfi I | Creative Enzymes

Sfi I

Cat :

ET-1173RE

Recognition Sequence :

GGCCNNNN↑NGGCC
CCGGN↓NNNNCCGG

Unit Definition :

One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 50°C in a total reaction volume of 50 μl.

Reaction Temperature :

50°C

Form :

Liquid

Storage Buffer :

10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.

Ligation :

After 10-fold overdigestion with enzyme more than 70% of the DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better.

Source :

An E.coli strain, that carries the cloned gene Sfi I from Streptomyces fimbriatus

Assayed on :

T7 DNA

Working buffer :

G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA

Non-specific hydrolisis :

No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 20 u.a. of enzyme for 16 hours at 50°C.

Size :

1000U; 5000U

Concentration, u.a./ml :

10000

Inactivation :

20min Under 65°C

Methylation sensitivity :

Blocked by overlapping Dcm methylation (CmCWGG): GGCCWGGNNGGCC.
Not blocked by overlapping Dcm methylation (CmCWGG): GGCCNNNNNGGCCWGG.

Reagents Supplied :

10 X SE-buffer G, BSA

Storage :

-20°C

Notes :

Incubation at 37°C rusults in 75-100% activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.

PDF

Our Products Cannot Be Used As Medicines Directly For Personal Use.

Inquiry

We are here to answer any question you may have

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies