Product Description :
Note! SetI cleaves a canonical site and several other sites with a weaker activity.
In the case of long incubation with SetI DNA can be digested to small oligos.
Recognition Sequence :
ASST↑
↓TSSA
Unit Definition :
One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the below indicated structure in 1 hour at 55°C in a total reaction volume of 20 μl.
Reaction Temperature :
55°C
Storage Buffer :
10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol.
Ligation :
After 5-fold overdigestion with enzyme, approximately 50% of the pBR322 DNA fragments can be ligated with T4 DNA Ligase and recut.
Source :
An E.coli strain that carries the cloned Set I gene from Streptomyces werraensis 37
Assayed on :
Double-stranded oligonucleotide
5`-CGAGTTTATAGCTGGGCCCAAC-3`
3`-GCTCAAATATCGACCCGGGTTG-5`
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Concentration, u.a./ml :
5000
Inactivation :
20min Under 80°C
Reagents Supplied :
10 X SE-buffer Y
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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