Sbf I

Cat :

ET-1170RE

Recognition Sequence :

CCTGCA↑GG
GG↓ACGTCC

Unit Definition :

One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Reaction Temperature :

37°C

Form :

Liquid

Storage Buffer :

10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol

Ligation :

After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these more than 90% canrecut.

Source :

An E.coli strain that carries the cloned Sbf I gene from Streptomyces species Bf61

Assayed on :

Lambda DNA

Working buffer :

Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Non-specific hydrolisis :

No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Size :

200U; 1000U

Concentration, u.a./ml :

5000

Inactivation :

20min Under 80°C

Reagents Supplied :

10 X SE-buffer Y

Storage :

-20°C

Notes :

High enzyme concentration may result in star activity.

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