Recognition Sequence :
G↑TAC
CAT↓G
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol.
Ligation :
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Source :
Rhodopseudomonas sphaeroides N
Working buffer :
B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
5000
Inactivation :
20min Under 80°C
Reagents Supplied :
10 X SE-buffer B
Our Products Cannot Be Used As Medicines Directly For Personal Use.
PDF