Recognition Sequence :
GCGAT↑CGC
CGC↓TAGCG
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Reaction Temperature :
55°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.
Ligation :
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Source :
Rhizoblum galegae
Assayed on :
Adenovirus-2 DNA
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 5 u.a. of enzyme for 16 hours at 55°C.
Concentration, u.a./ml :
5000-10000
Inactivation :
20min Under 80°C
Methylation sensitivity :
Not blocked by overlapping Dam-methylation (GmATC): GCGATCGC
Blocked by CpG methylation.
Reagents Supplied :
10 X SE-buffer Y
Notes :
High enzyme concentration may result in star activity.
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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