Recognition Sequence :
TTA↑TAA
AAT↓ATT
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol
Ligation :
After 5-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated and of these 95% can be recut.
Source :
Pseudomonas species-SE-G49
Working buffer :
B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
5000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer B
Notes :
The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25.
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