Pct I

Cat :

ET-1147RE

Recognition Sequence :

GAATGCN↑
CTTAC↓GN

Unit Definition :

One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Reaction Temperature :

37°C

Form :

Liquid

Storage Buffer :

10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; and 50% glycerol.

Ligation :

After 10-fold overdigestion with enzyme >90% of the DNA fragments can be ligated and recut.

Source :

Planococcus citreus SM

Assayed on :

Lambda DNA

Working buffer :

O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Non-specific hydrolisis :

No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Size :

1000U; 5000U

Concentration, u.a./ml :

10000

Inactivation :

20min Under 65°C

Reagents Supplied :

10 X SE-buffer O

Storage :

-20°C

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