Recognition Sequence :
GCTCTTCN↑
CGAGAAG(N)4↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA and 50% glycerol
Ligation :
After 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and 95% of these can be recut.
Source :
Planococcus citreus S
Working buffer :
B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 0.5 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
500-2000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer B
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