Recognition Sequence :
A↑CATGT
TGTAC↓A
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol
Ligation :
After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Source :
An E.coli strain that carries the cloned Pci I gene from Planococcus citreus SE-F45
Working buffer :
O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
10000
Inactivation :
20min Under 65°C
Methylation sensitivity :
Not blocked by ACmATGT methylation.
Blocked by mACATGT methylation.
Reagents Supplied :
10 X SE-buffer O
Notes :
The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13.
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