Recognition Sequence :
TCG↑CGA
AGC↓GCT
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol;
Ligation :
After 5-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated and 90% of them can be recut. In the presence of 10% PEG ligation is better.
Assayed on :
Lambda DNA (dam-)
Working buffer :
W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
5000
Inactivation :
20min Under 80°C
Methylation sensitivity :
Blocked by overlapping Dam methylation (GmATC): TCGCGATC.
Reagents Supplied :
10 X SE-buffer W
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