Mutation Detection Kit

Cat :

CE-3508

Product Description :

Mutation Detection Kit provides a simple, reliable, and rapid method for the detection of site specific cleavage of genomic DNA that is extracted from cells transfected with constructs expressing engineered nucleases such as Transcription activator-like effector nucleases (TALEN), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, or Zinc-finger nucleases (ZFN). Mutation Detection Kit includes High-Fidelity DNA polymerase for amplifying the target regions from cells, and T7 Endonuclease I for recognizing and detecting the mismatches caused by gene editing tools. It provides an easy and reliable approach for estimating the efficiency of genome editing.
Components
High-Fidelity DNA polymerase
5X PCR Reaction Buffer
T7 Endonuclease I
10X GenCrispr T7 Endonuclease I Reaction buffer
Control Template DNA
Control Primer Mix
Protease K
dNTP

Storage Buffer :

10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.4, at 25°C

Source :

E.coli

Size :

25 reactions/kit

Storage :

Store at -20 ℃

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