Recognition Sequence :
CC↑NGG
GGN↓CC
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoetyanol; 100 μg/ml BSA; and 50% glycerol
Ligation :
After 2-fold MspR9 I overdigestion >5% of the DNA fragments can be ligated and recut.
Source :
Micrococcus species R9
Assayed on :
Lambda DNA (dcm-)
Working buffer :
O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
20000
Inactivation :
20min Under 80°C
Methylation sensitivity :
Blocked by overlapping Dcm methylation(CmCWGG): CCAGG and CCTGG.
Reagents Supplied :
10 X SE-buffer O
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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