Product Description :
mRNA Cap 2'-O-Methyltransferase can use S-adenosylmethionine (SAM) as a methyl donor, adding methyl to the 2'-O of the first nucleotide next to the cap structure at the 5'end of the RNA Group, the Cap 0 is methylated to Cap 1. This enzyme can only act on RNA with a 7-methylguanosine cap structure (m7GpppN), and will not act on RNA with pN, ppN, pppN or GpppN at the 5'end. The Vaccinia Capping System can be used to prepare RNA with a 7-methylguanosine cap structure (m7GpppN).
Components :
·mRNA Cap 2'-O-Methyltransferase
Unit Definition :
The amount of enzyme required to methylate 10 pmol of 80 nt oligonucleotide transcript with m7Gppp cap structure in 1 hour at 37°C, pH 8.0 is defined as 1 activity unit.
Storage Buffer :
50 mM Tris-HCl(25℃, pH 8.0), 5 mM KCl, 1 mM MgCl2, 1 mM DTT
Concentration, u.a./ml :
50U/μl
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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