Product Description :
mRNA Cap 2'-O-Methyltransferase can use S-adenosylmethionine (SAM) as a methyl donor, adding methyl to the 2'-O of the first nucleotide next to the cap structure at the 5'end of the RNA Group, the Cap 0 is methylated to Cap 1. This enzyme can only act on RNA with a 7-methylguanosine cap structure (m7GpppN), and will not act on RNA with pN, ppN, pppN or GpppN at the 5'end. The Vaccinia Capping System can be used to prepare RNA with a 7-methylguanosine cap structure (m7GpppN).
Components :
• mRNA Cap 2'-O-Methyltransferase (50 U/μl)
• 10 x Capping Buffer
• SAM (32 mM)
• RNase-Free Water
Unit Definition :
1 Unit is defined as the amount of enzyme (m7GpppN) required to methylate 10 nmol of 80 nucleotides (80 nt) RNA with a 7-methylguanosine cap structure (m7GpppN) within 1 hour under the conditions of 37°C and pH 8.0
Storage Buffer :
20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol and 0.1% Triton X-100(25℃, pH 8.0)
Concentration, u.a./ml :
50 U/μl
Our Products Cannot Be Used As Medicines Directly For Personal Use.
Protocol