Mly113 I

Cat :

ET-1134RE

Recognition Sequence :

GG↑CGCC
CCGC↓GG

Unit Definition :

One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Reaction Temperature :

37°C

Form :

Liquid

Storage Buffer :

10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol

Ligation :

After 3-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.

Source :

Micrococcus lylae 113

Assayed on :

T7 DNA

Working buffer :

B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)

Non-specific hydrolisis :

No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C.

Size :

200U; 1000U

Concentration, u.a./ml :

2000

Inactivation :

20min Under 65°C

Reagents Supplied :

10 X SE-buffer B

Storage :

-20°C

Notes :

High enzyme concentration may result in star activity.

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