Product Description :
McrBC specifically cleaves DNA containing methyl cytosine (mC: 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu: A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40–2,000 bp.
Unit Definition :
One unit is defined as the amount of enzyme required to cleave 0.25 μg of pBR322 containing a single McrBC site (pBR322-mu-BamHI-MA/ul) in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl, pH 7.5 (at 25℃); 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 0.05％ BSA; 50％ Glycerol
Source :
recombinant proteins expressed in E. coli
Concentration, u.a./ml :
1 U/μl
Reagents Supplied :
10X McrBC Buffer 500 μl; 0.1% BSA 1 ml; 100 mM GTP（100X） 50 μl
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