Product Description :
M-MLV Reverse Transcriptase(RNase H-) is a modified and optimized M-MLV reverse transcriptase, which can use RNA or DNA as a template to synthesize complementary DNA strands in the presence of primers, that is, the first strand of cDNA (complementary DNA) can be synthesized. . This product is one of the most commonly used reverse transcriptases. It is widely used to synthesize the first strand of cDNA after obtaining total RNA or mRNA. It can be subsequently used for PCR and real-time PCR, also known as quantitative PCR (quantitative PCR, qPCR). , cDNA second-strand synthesis and cDNA library construction. This product has no RNase H activity, high reverse transcription efficiency and long product length. Compared with wild-type M-MLV reverse transcriptase, this product has no RNase H activity and cannot selectively cleave RNA in RNA and DNA hybrid duplexes, thus contributing to the efficient synthesis of longer cDNA. This product has been tested to easily complete reverse transcription of genes up to 8 kb in length, and the maximum length of reverse transcription can exceed 10 kb.
Components :
·M-MLV Reverse Transcriptase(RNase H-)
·5X Reaction Buffer
Unit Definition :
One unit of the enzyme incorporates 1 nmol of dTTP into acid-precipitable material in 10 min at 37°C using poly(A)·oligo(dT) 12-18 as template-primer.
Storage Buffer :
20 mM Tris-HCl (pH 7.5), 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01% NP-40 and 50% glycerol
Concentration, u.a./ml :
200U/μl
Inactivation :
Incubating at 80°C for 10 minutes can cause inactivation of M-MLV reverse transcriptase (RNase H-); chelating agents such as EDTA and EGTA, inorganic phosphate or pyrophosphate, and polyamine can inhibit M-MLV reverse transcriptase. (RNase H-) has inhibitory effect.
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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