Recognition Sequence :
↑GATC
CTAG↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol.
Ligation :
After 3-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Source :
Kurthia zopfii 9
Working buffer :
G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
1000-5000
Inactivation :
20min Under 65°C
Methylation sensitivity :
Not blocked by overlapping Dam methylation (GmATC): GATC.
Blocked by overlapping CG methylation: CGATmCG.
Cleaved of DNA is impaired by overlapping CG methylation: GATmCG.
Reagents Supplied :
10 X SE-buffer G
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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