Product Description :
The transfer buffer is white to almost white instant granules. Each bag of Transfer Buffer (Semi Dry) instant granules can produce 1 L 1× Transfer Buffer (Semi Dry) buffer, which is easy to operate and easy to use. The main components of Transfer Buffer (Semi Dry) are 48 mM Tris, 39 mM glycine and 0.04% SDS.
Transfer Buffer (Semi Dry) is suitable for Western Blot semi-dry electrophoresis transfer membrane. Pre-mixed with SDS can be applied to large molecular weight proteins or strong hydrophobic proteins.
Protocol :
1. Measure about 600 ml of distilled water into the beaker, and place a magnetic stir bar in the beaker.
2. Place the beaker on a magnetic stirrer, slowly add the entire contents of 1 bag of Transfer Buffer(Semi Dry) instant granules, and stir the solution until it is completely dissolved.
3. Add distilled water to the Transfer Buffer(Semi Dry) solution in step 2, and dilute to 1 L, which is 1× buffer.
PDF