Product Description :
PBS is white to almost white instant granules. Each bag of PBS instant granules can produce with 1 L of 1×PBS buffer, which is easy to operate and easy to use. The main components of PBS are sodium chloride, potassium chloride and phosphate. In 1× PBS buffer, the concentration of sodium chloride is 137 mM, the concentration of potassium chloride is 2.7 mM, and the concentration of phosphate is 10 mM.
PBS is a buffer widely used in biology and can be used for immunoassay experiments, microbiology experiments, protein biochemical experiments, etc. The PBS buffer prepared with PBS instant particles can be used in cell culture, washing and other related experiments after being treated with a 0.22 μm sterilizing filter.
Protocol :
1. Measure about 600 ml of distilled water into the beaker, and place a magnetic stir bar in the beaker.
2. Place the beaker on a magnetic stirrer, slowly add the entire contents of 1 bag of PBS instant granules, and stir the solution until it is completely dissolved.
3. Add distilled water to the PBS solution in step 2, and dilute to 1 L, which is 1× buffer.
After being formulated into 1× solution, the pH value is 7.4±0.2@25℃.
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