Recognition Sequence :
G↑CGC
CGC↓G
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Ligation :
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Source :
An E.coli strain that carries the cloned HspA I gene from Haemophilus species A1
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
20000
Inactivation :
20min Under 80°C
Methylation sensitivity :
Blocked by CG methylation 5'-G(5mC)GC-3'/3'-CG(5mC)G-5'.
Not blocked by methylation 5'-GCG(5mC)-3'/3'-CGCG-5'.
Cut hemimethylated site: 5'- G(5mC)GC-3'/3'-CGCG-5`
Reagents Supplied :
10 X SE-buffer Y
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