Hpa I

Cat :

ET-1121RE

Recognition Sequence :

GTT↑AAC
CAA↓TTG

Unit Definition :

One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Reaction Temperature :

37°C

Form :

Liquid

Storage Buffer :

10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol.

Ligation :

After 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut.

Source :

An E.coli strain, that carries the cloned gene Hpa I from Haemophilus parainfluenzae

Assayed on :

Lambda DNA

Working buffer :

Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Non-specific hydrolisis :

No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.

Size :

500 U; 2500U

Concentration, u.a./ml :

5000

Inactivation :

20min Under 65°C

Reagents Supplied :

10 X SE-buffer Y.

Storage :

-20°C

Notes :

High enzyme concentration results in star activity.

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