Hind III | Creative Enzymes

Hind III

Cat :

ET-1119RE

Recognition Sequence :

A↑AGCTT
TTCGA↓A

Unit Definition :

One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Reaction Temperature :

37°C

Form :

Liquid

Storage Buffer :

10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol

Ligation :

After 50-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.

Source :

An E.coli strain, that carries the cloned gene Hind III from Haemophilus influenzae Rd

Assayed on :

Lambda DNA

Working buffer :

W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Non-specific hydrolisis :

No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Size :

5000U; 25000U

Concentration, u.a./ml :

20000; 50000

Inactivation :

20min Under 80°C

Reagents Supplied :

10 X SE-buffer W, BSA

Storage :

-20°C

Notes :

To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.

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