Recognition Sequence :
GACGC(N)5↑
CTGCG(N)10↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of DNA pBR322 in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol
Ligation :
After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Source :
An E.coli strain that carries the cloned HgaI gene from Haemophilus gallinarum
Working buffer :
B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Non-specific hydrolisis :
Incubation with > 2 units of HgaI per 1 μg of DNA and digestion > 1 hour are not recommended.
Concentration, u.a./ml :
1000
Inactivation :
20min Under 65°C
Methylation sensitivity :
Blocked by CG methylation.
Reagents Supplied :
10 X SE-buffer B
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