Recognition Sequence :
GC↑NGC
CGN↓CG
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 200ug/ml BSA; 1mM DTT; 50% glycerol
Ligation :
After 5-fold overdigestion with enzyme about 5% of the DNA fragments can be ligated and recut.
Source :
An E.coli strain that carries the cloned Fsp4H I gene from Flavobacterium species 4H
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
3000-5000
Inactivation :
20min Under 65°C
Methylation sensitivity :
Blocked by methylation: 5`-G(5mC)NGC-3`/ 3`-CGN(5mC)G-5`.
Reagents Supplied :
10 X SE-buffer Y
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