Recognition Sequence :
GGATG(N)9↑
CCTAC(N)13↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol
Ligation :
After 2-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Source :
An E.coli strain that carries the cloned Fok I gene from Flavobacterium okeanokoites
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 1 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
1000-2000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer Y
Notes :
Overdigestions of > 5 units of Fok I per 1μg of DNA and incubations > 2 hours are not recommended.
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